Journal:

Article Title: Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from Fc?RII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression

doi: 10.1128/JVI.77.21.11332-11346.2003

Figure Lengend Snippet: MV-N binding on different cell types

Article Snippet: The cell lines 3D1 (murine cortical TEC), HeLa (epithelial human carcinoma from cervix), MeWo (fibroblastic human melanoma from lymph node), NIH 3T3 (mouse fibroblasts from embryo), Jurkat (human acute T-cell leukemia), and Vero (monkey fibroblast from kidney) were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques: Binding Assay, Inhibition, Expressing

Journal: Clinical and Developmental Immunology

Article Title: Modulatory Effect of 1,25-Dihydroxyvitamin D 3 on IL1 β -Induced RANKL, OPG, TNF α , and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A

doi: 10.1155/2013/160123

Figure Lengend Snippet: Effect of 1,25(OH) 2 D 3 on IL β -induced TNF α and IL-6 production in MH7A. MH7A cells were stimulated with 20 ng/mL IL1 β and then treated with different concentrations of 1,25(OH) 2 D 3 (0.1 nM, 1 nM, 10 nM, and 100 nM) for 48 h. The effects of 1,25(OH) 2 D 3 on IL β -induced TNF α (a) and IL-6 (b) mRNA expression in MH7A was analyzed by real-time PCR, and the production of IL-6 in supernatants was shown in (c). The data shown are the mean ± SD for three independent experiments, each in triplicate. * P < 0.05 compared to cells cultured with IL-1 β alone. ** P < 0.01 compared to cells cultured with IL-1 β alone.

Article Snippet: Cytokine production was determined in cell culture supernatants using ELISA specific for human IL-6 and TNF α (BOSTER-BIO; Wuhan; China) following manufacturer's guidelines.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

Journal: bioRxiv

Article Title: Tissue-wide profiling of human lungs reveals spatial sequestration of macrophages in tuberculosis

doi: 10.1101/2025.04.11.648467

Figure Lengend Snippet: (A) Heatmap showing the relative abundance of CD4 + (top) and CD8 + (bottom) T cell sub-populations in each lesion type. Rows are ordered based on the similarity in marker expression patterns. N, the border of necrotic lesions. (B) Representative OPAL multi-plex image illustrating a germinal center-like structure in a B3-type lesion. The image with merged DAPI, CD20, BCL6, and PNAd is shown. Arrows indicate PNAd + high endothelial venules and arrow heads indicate BCL6 + B cells. (C) Paired analysis comparing the spatial association relationships between B cells and Tfh (C10) or proinflammatory CD4 + T cells (C08) in all B3-type lesions. Each line indicates a lesion. The level of statistical significance is determined using Wilcoxon signed-rank test, **** P < 0.0001. (D) Cell type pheno-map of a representative B3-type lesion with cell type indicated. (E) Scatter plots showing the spatial centrality index (immCPI) of Tfh (C10), proinflammatory CD4 + T cells (C08), and proinflammatory CD8 + T cells (C11) in all B3-type lesions ( n = 110). The level of statistical significance is determined using a Mann-Whitney U test, **** P < 0.0001. (F) Heatmap showing the median value of pair-wised spatial association relationships between CD4 + and CD8 + T cell subsets in B3 (left) and T (right) type lesions. (G) Column scatter plot showing spatial relationship between macrophages and CD4 + and CD8 + T cell subtypes in T-type lesions. (H) Heatmap indicating statistical significance from post hoc multi-group comparison results in (G) using a One-way ANOVA test, the level of significance was set at P = 0.05.

Article Snippet: Next, tissue sections were incubated for 1 hour at room temperature with primary antibodies against CD20 (1:100, L26, Abcam), CD4 (1:100, EPR6855, Abcam), and overnight under 4°C with BCL6 (1:100, EPR11410-43, Abcam), and PNAd (1:200, MECA-79, Novus Biologicals).

Techniques: Marker, Expressing, MANN-WHITNEY, Comparison